Experimental model of Achilles tendon injury in laboratory rats
Background. Achilles tendon injury occurs in persons leading an active lifestyle, or professionally engaged in sport. Chronic inflammation and damage to the Achilles tendon result in sustained and longtime disability, as well as to significant expenditures for treatment. The purpose of the study was to develop and verify an experimental biological model of damage to the Achilles tendon in laboratory rats using magnetic resonance imaging. Materials and methods. Experimental modeling was performed in 10 laboratory rats aged up to 12 months, weighing up to 300–350 g. Simulation of tendon calvaneus injury in white laboratory rats was performed surgically by arthrotomy, under aseptic conditions, under anesthesia. In order to study the changes taking place in the injured tendon, on the 7th, 14th, 21st, and 28th days after the surgical intervention, the rats underwent magnetic resonance imaging (MRI) of the zone of the damaged Achilles tendon. Results. As a result of the surgical interventions, one rat was found to have no complications, either during surgery or during the postoperative period. All postoperative wounds healed with primary tension. Accordanly, all rats passed the complete protocol of the study. It was established that the inflammatory process in the Achilles tendon lasts for 21 days postoperatively, and the restoration of the tendon tissue defect depends on the reparative potential of an individual. Conclusions. The experimental biological model of Achilles tendonpathy has been developed and verified, that can be used to study the effectiveness of various types of treatment.
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