Experimental model of Achilles tendon injury in laboratory rats
Background. Achilles tendon injury occurs in persons leading an active lifestyle, or professionally engaged in sport. Chronic inflammation and damage to the Achilles tendon result in sustained and longtime disability, as well as to significant expenditures for treatment. The purpose of the study was to develop and verify an experimental biological model of damage to the Achilles tendon in laboratory rats using magnetic resonance imaging. Materials and methods. Experimental modeling was performed in 10 laboratory rats aged up to 12 months, weighing up to 300–350 g. Simulation of tendon calvaneus injury in white laboratory rats was performed surgically by arthrotomy, under aseptic conditions, under anesthesia. In order to study the changes taking place in the injured tendon, on the 7th, 14th, 21st, and 28th days after the surgical intervention, the rats underwent magnetic resonance imaging (MRI) of the zone of the damaged Achilles tendon. Results. As a result of the surgical interventions, one rat was found to have no complications, either during surgery or during the postoperative period. All postoperative wounds healed with primary tension. Accordanly, all rats passed the complete protocol of the study. It was established that the inflammatory process in the Achilles tendon lasts for 21 days postoperatively, and the restoration of the tendon tissue defect depends on the reparative potential of an individual. Conclusions. The experimental biological model of Achilles tendonpathy has been developed and verified, that can be used to study the effectiveness of various types of treatment.
Full Text:PDF (Українська)
Mafulli N. Achilles tendinopathy: etiology and management / Mafulli N., Sharma P., Luscombe K.L. // Journal of Social Medicine. — 2004. — Vol. 97(10). — P. 472-476.
Alfredson H. A treatment algorithm for managing Achilles tendinopathy: new treatment options / Alfredson H., Cook J. // British Journal of Sport Medicine. — 2007. — Vol. 41. — P. 211-216.
Sen B. The effect of autologous platelet rich plasma in the treatment of Achilles tendon ruptures: an experimental study on rabbits / Sen B., Guler S., Cecen B., Kumptepe E. // Balkan Medical Journal. — 2016. — Vol. 33. — P. 94-101.
Kannus P. Tendons — a source of major concern in competitive and recreational athletes // Scand. J. Med. Sci. Sports. — 1997. — 7. — Р. 53-54.
Silva R.D., Glazebrook M.A., Campos V.C., Vasconcelos A.C. Achilles tendinosis: a morphometrical study in a rat model // Int. J. Clin. Exp. Pathol. — 2011. — 4. — Р. 683-691.
Ng G.Y., Chung P.Y., Wang J.S., Cheung R.T. Enforced bipedal downhill running induces Achilles tendinosis in rats // Connect. Tissue Res. — 2011. — 52. — Р. 466-471
Bell R., Li J., Gorski D.J. et al. Controlled treadmill exercise eliminates chondroid deposits and restores tensile properties in a new murine tendinopathy model // J. Biomech. — 2013. — 46. — Р. 498-505.
Andersson G., Backman L.J., Scott A. et al. Substance P accelerates hypercellularity and angiogenesis in tendon tissue and enhances paratendinitis in response to Achilles tendon overuse in a tendinopathy model // Br. J. Sports Med. — 2011. — 45. — Р. 1017-1022.
Experimental model of Achilles tendon injury in rats Flavia Emi Akamatsu I, Samir Omar Saleh II, Walcy Rosolia Teodoro III, Alexandre Queiroz da Silva I // Acta Cirúrgica Brasileira. — 2014. — Vol. 29(7). — Р. 417.
Xiong X., Wu L., Xiang D., Ni G., Zhao P., Yu B. Effect of platelet-rich plasma injection on early healing of Achilles tendon rupture in rats // Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. — 2012 Apr. — 26(4). — Р. 466-71.
Copyright (c) 2017 TRAUMA
This work is licensed under a Creative Commons Attribution 4.0 International License.
© Publishing House Zaslavsky, 1997-2020