Study of knee joint structural changes in rabbit osteoarthritis models and intra-articular injections of platelet rich plasma and bone marrow aspirate concentrate
Keywords:osteoarthritis, knee joint, morphometry, platelet rich plasma, bone marrow aspirate concentrate
Background. The article presents the results of an experimental study on the knee joint structural changes and morphometric evaluation of articular cartilage with the injections of platelet rich plasma and bone marrow aspirate concentrate. Materials and methods. Cartilage defect creation, anterior cruciate ligament intersection and medial meniscus resection were performed for the induction of osteoarthritis in rabbits. After 28 days, 0.9% NaCl, or platelet rich plasma, or bone marrow aspirate concentrate was administered intra-articularly; 0.9% NaCl or platelet rich plasma was injected intra-articularly in 14 days. After 2 months, histological and morphometric examination of the epiphyseal surface of the femur and tibia was performed. Results. Animal models showed identical diameter of epiphyseal cartilage defect, chondrocyte reduction (by 55.1 %), reduction of periphocal articular surface thickness (by 53.2 %) and relative bone density of epiphysis (by 48.5 %). Modeling of the joint defect was not limited to the area of damage and caused degenerative changes of the articular cartilage of the tibia. Statistical analysis showed that the introduction of platelet rich plasma had no preventive effect on the degenerative changes in the articular surface. In the bone marrow aspirate concentrate group, the thickness of the hyaline cartilage of the tibia was found to be 15.3 % (P < 0.05) greater compared with the 0.9% NaCl group and the double platelet rich plasma group. However, the relative density of subepiphyseal bone tissue in the experimental groups with osteoarthritis had no differences. Conclusions. The effect of platelet rich plasma and bone marrow aspirate concentrate on knee joint structural changes in rabbit osteoarthritis models was investigated. Statistically significant preservation of the tibial cartilage height in the group of animals using bone marrow aspirate concentrate/platelet rich plasma was demonstrated.
Gregory M.H., Capito N., Kuroki K., Stoker A.M., Cook J.L., Sherman S.L. A review of translational animal models for knee osteoarthritis. Arthritis. 2012. 2012. 764621. doi: 10.1155/2012/764621.
Helito C.P., Bonadio M.B., Sobrado M.F. et al. Comparison of Floseal® and Tranexamic Acid for Bleeding Control after Total Knee Arthroplasty: a Prospective Randomized Study. Clinics (Sao Paulo). 2019. 74. e1186. Published 2019 Nov 25. doi: 10.6061/clinics/2019/e1186.
Rughetti A., Giusti I., D’Ascenzo S., Leocata P., Carta G., Pavan A., Dell’Orso L., Dolo V. Platelet gel-released supernatant modulates the angiogenic capability of human endothelial cells. Blood Transfus. 2008. 6(1). Р. 12-17.
Fleming B.C., Proffen B.L., Vavken P., Shalvoy M.R., Machan J.T., Murray M.M. Increased platelet concentration does not improve functional graft healing in bio-enhanced ACL reconstruction. Knee Surg Sports Traumatol Arthrosc. 2015. 23(4). Р. 1161-70.
Campbell K.A., Saltzman B.M., Mascarenhas R., Khair M.M., Verma N.N., Bach B.R. Jr, Cole B.J. Does intra-articular platelet-rich plasma injection provide clinically superior outcomes compared with other therapies in the treatment of knee osteoarthritis? A systematic review of overlapping meta-analyses. Arthroscopy. 2015. 31(10). Р. 2036-45.
Moatshe G., Morris E.R., Cinque M.E. et al. Biological treatment of the knee with platelet-rich plasma or bone marrow aspirate concentrates. Acta Orthop. 2017. 88(6). Р. 670-674. doi: 10.1080/17453674.2017.1368899.
Krych A.J., Nawabi D.H., Farshad-Amacker N.A., Jones K.J., Maak T.G., Potter H.G., Williams R.J. 3rd. Bone marrow concentrate improves early cartilage phase maturation of a scaffold plug in the knee: A comparative magnetic resonance imaging analysis to platelet-rich plasma and control. Am. J. Sports Med. 2016. 44(1). Р. 91-8.
Ткачук П.В., Страфун С.С., Кучменко О.Б. и др. Оценка влияния тромбоцитарной плазмы на биохимические показатели крови в экспериментальной модели остеоартроза коленного сустава. Травма. 2019. 20(4). C. 39-45.
Liu Z., Hu X., Man Z., Zhang J., Jiang Y., Ao Y. A novel rabbit model of early osteoarthritis exhibits gradual cartilage degeneration after medial collateral ligament transection outside the joint capsule. Sci. Rep. 2016. 6. 34423. Published 2016 Oct 19. doi: 10.1038/srep34423.
Copyright (c) 2020 TRAUMA
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our edition uses the copyright terms of Creative Commons for open access journals.
Authors, who are published in this journal, agree with the following terms:
- The authors retain rights for authorship of their article and grant to the edition the right of first publication of the article on a Creative Commons Attribution 4.0 International License, which allows others to freely distribute the published article, with the obligatory reference to the authors of original works and original publication in this journal.
- Directing the article for the publication to the editorial board (publisher), the author agrees with transmitting of rights for the protection and using the article, including parts of the article, which are protected by the copyrights, such as the author’s photo, pictures, charts, tables, etc., including the reproduction in the media and the Internet; for distributing; for the translation of the manuscript in all languages; for export and import of the publications copies of the writers’ article to spread, bringing to the general information.
- The rights mentioned above authors transfer to the edition (publisher) for the unlimited period of validity and on the territory of all countries of the world.
- The authors guarantee that they have exclusive rights for using of the article, which they have sent to the edition (publisher). The edition (the publisher) is not responsible for the violation of given guarantees by the authors to the third parties.
- The authors have the right to conclude separate supplement agreements that relate to non-exclusive distribution of their article in the form in which it had been published in the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.
- The policy of the journal permits and encourages the publication of the article in the Internet (in institutional repository or on a personal website) by the authors, because it contributes to productive scientific discussion and a positive effect on efficiency and dynamics of the citation of the article.
- The rights to the article are deemed transferred by the authors to the edition (the publisher) since the moment of the publication of the article in the printed or electronic version of journal.